Anti-FHL2 (Human) mAb

WB - 1-5 ug/mL IP - 3 ug / 200-300 uL of cell extract IHC - 1-5 ug/mL Heat treatment is necessary for paraffin embedded sections.

Proteins containing LIM domains (which are double zinc finger motifs implicated in protein binding) are important regulators of cell growth, cell differentiation, and remodeling of the cell cytoskeleton. Human Four-and-a-half LIM-only protein 2 (FHL2), also known as DRAL/Slim3 is a 32 kDa protein expressed predominantly in human heart and to a lesser extent in skeletal muscle, testis, and prostate epithelium. Since FHL2 is abundant in heart tissue, it may play a role in the regulation of myofibrillogenesis of heart via LIM-domain binding to focal adhesions. FHL2 has also been identified as a co-activator of the androgen receptor where it promotes androgen receptor transcriptional activity. Stimulation of the Rho signaling pathway induces translocation of FHL2 to the nucleus and subsequent activation of FHL2- and androgen receptor-dependent genes. FHL2 also acts as a trancriptional repressor in muscle cells and is involved in modulation of β-catenin-dependent transcription of Wnt-responsive genes.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.

Human: 2274 Mouse: 14200

Full-length recombinant human FHL2

This antibody reacts with mouse FHL2.

1 year

This antibody was purified from hybridoma (clone 11-134) supernatant using protein-A Sepharose. This hybridoma was established by fusion of mouse myeloma cell SP2/0-Ag4 with Balb/c mouse splenocyte immunized with His-FHL2.

FHL2