Anti-TLR9 (CD289) (Human) mAb

FCM - 10 ug/mL (final concentration)

Toll, a Drosophila receptor molecule with extracellular leucine-rich repeat (LRR), has a role in triggering innate defense against bacteria or fungi. Toll-like receptor 9 (TLR9) is a member of TLR family. Members of the TLR family are components of the mammalian anti-microbial response, signaling with a domain closely related to that of IL-1 receptors. Recognition by innate immune cells of the pathogen associated molecular patterns (PAMPs) bacterial CpG-DNA depends on TLR9. TLR9 is dispersedly and intracellularily expressed in resting cells. Upon CpG ODN treatment, TLR9 is recruited to endosome and initiates signal transduction, which activates mitogen-activated protein kinases (MAPKs) and NF-?B through the MyD88/IRAK/TRAF6 kinases cascade.

1 mg/mL

1.0 mg/mL in PBS/50% glycerol, pH 7.2

Human: 54106 Mouse: 81897

Recombinant human TLR9 (1-815 a.a.)

This antibody reacts with mouse TLR9 on Flow cytometry. Reactivity of 5G5 to endogenous human TLR9 is not confirmed in our laboratory. However, it is reported that this clone reacts with human TLR9 in reference number 2).

1 year

This antibody was purified from culture supernatant using protein A agarose. This hybridoma (clone 5G5) was established by fusion of mouse myeloma cell P3X with C57BL/6 mouse splenocyte immunized with the recombinant human TLR9 (1-815 aa).

TLR9