Anti-Glutamic Acid Decarboxylase (GAD) (Human) mAb
| Code | Size | Price |
|---|
| MBL-M018-3 | 100 ug | £323.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1 κ
Antibody Clonality: Monoclonal
Antibody Clone: 9A6
Regulatory Status: RUO
Target Species:
- Mouse
- Rat
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1 ug/mL (chemiluminescence detection system) IHC - 1-10 ug/mL
Background:
Glutamic acid decarboxylase (GAD) catalyzes formation of γ-Aminobutyric acid (GABA), which is an inhibitory neurotransmitter, and is present in brain as well as several tissues outside the central nervous system. Biological functions of GAD and GABA extend beyond regulation of neurotransmission to include effects on the immune system as well as modulation of cell proliferation, protein synthesis, and metabolism. Two isoforms of GAD, GAD65 and GAD67, each derived from a single separate gene, were isolated from human fetal brain λgt11 cDNA library.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul PBS containing 50% glycerol, pH 7.2. Contains no preservatives.
Gene IDs:
Human: 2571 Mouse: 14415 Rat: 24380
Immunogen Translated:
Full-length recombinant human GAD65 (1-585 a.a.)
Reactivity:
This antibody reacts with GAD65 and
GAD67 on Western blotting and Immunohistochemistry in
murine and rat samples. This antibody detects 65 kDa and
67 kDa GAD by Western blotting using mouse and rat total
brain cell lysate.
Shelf Life:
1 year
Source:
This antibody was purified from mouse ascites
fluid using protein A agarose. This hybridoma was
established by fusion of mouse myeloma cell P3U1 with
Balb/c mouse splenocyte immunized with the recombinant
GST-human brain GAD65 fusion protein corresponding to
full-length amino acids (1-585 a.a.).
Target:
GAD
References
1) Kuwajima, T. et al. J. Neurosci. 26, 5383-5392 (2006)
2) Kasuga, A. et al. J. Autoimmunity 9, 105-111 (1996)
3) Daw, K. et al. J. Immunol. 156, 818-825 (1996)
4) Grubin, C. E. et al. Diabetologia 37, 344-350 (1994)
5) Mauch, L. et al. J. Biochem. 113, 699-704 (1993)
6) Deaizpurua, H. J. et al. Diabetes 41, 1182-1187 (1992)
7) Bu, D-F. et al. PNAS. 89, 2115-2119 (1992)
8) Michelsen, B. K. et al. PNAS. 88, 8754-8758 (1991)
9) Karlsen, A. E. et al. PNAS. 88, 8337-8341 (1991)
Clone 9A6 is used in reference number 1).