Anti-Neuropsin (Mouse) mAb
| Code | Size | Price |
|---|
| MBL-M021-3 | 100 ug | £323.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: MabB5
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Neutralisation
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1 ug/mL (chemiluminescence detection system) IP - It is reported that MabB5 can be used in this application in the reference number 4 and 6. ICC - It is reported that MabB5 can be used in this application in the reference number 3). IHC - It is reported that MabB5 can be used in this application in the reference number 7). NT - It is reported that MabB5 can be used in this application in the reference number 1) and 2).
Background:
Neuropsin is a trypsin-type, extracellular, serine protease that was first cloned from mouse limbic brain as a factor related to neural plasticity. Physiological studies indicated that kindling stimulation induced neuropsin mRNA and its protease activity also increased in limbic areas during kindling and long-term potentiation. Subsequent study indicated that neuropsin mRNA localized not only in the limbic brain, but also in developing organs and adult keratinocytes. Recent immunohistochemical analysis demonstrated that neuropsin localized exclusively in the keratinizing epithelia of the epidermis and various stratified mucous membranes. During development, neuropsin was found in the various epithelia at embryonic days 14.5-15.5, prior to formation of the stratum corneum. Much stronger expression of neuropsin in nude mouse skin and mucous membranes than in normal mice. Those studies suggested that neuropsin is related to differentiation of keratinocytes and the process of keratogenesis.
Formulation:
This antibody is lyophilized form. Prepare a stock solution by dissolving the lyophilized antibody with 100 mL of distilled water. After reconstitution, IgG concentration should be 1 mg/mL in PBS (pH 7.2)/1% Sucrose. No preservative is contained.
Gene IDs:
Human: 221391 Mouse: 353344
Immunogen Translated:
Recombinant protein
Reactivity:
This antibody reacts with mouse
neuropsin (32 kDa) on Western blotting with total cell
lysate from neuropsin overexpressed insect cell.
Shelf Life:
1 year
Source:
This antibody was purified from rat ascites
fluid using protein A agarose. This hybridoma (clone
MabB5) was established by fusion of rat myeloma cell
Y3Ag1.2.3 with Wister rat splenocyte immunized with the
recombinant protein.
Target:
Neuropsin
References
1) Tamura, H., et al., J. Physiol. 570, 541-551 (2006)
2) Matsumoto-Miyai, K., et al., J. Neurosci. 23, 7727-7736 (2003)
3) Oka, T., et al., J. Biol. Chem. 277, 14724-14730 (2002)
4) Kato, K., et al., J. Biol. Chem. 276, 14562-14571 (2001)
5) Momota, Y., et al., Eur. J. Neurosci. 10, 760-764 (1998)
6) Shimizu, C., et al., J. Biol. Chem. 273, 11189-11196 (1998)
7) Inoue, N., et al., J. Invest. Dermatol. 110, 923-931 (1998)
8) Chen, Z. L., et al., J. Histochem. Cytochem. 46, 313-320 (1998)
9) Okabe, A., et al., Brain Res. 728, 116-120 (1996)
10) Chen, Z. L., et al., J. Neurosci. 15, 5088-5097 (1995)
Clone MabB5, also known as mAbB5 or B5mAb, is
used in reference number 1) - 7).