Anti-Caspase-4 (Human) mAb

WB - 1 ug/mL (chemiluminescence detection system)

The interleukin-1β converting enzyme (ICE)/CED-3 family proteases has been implicated in playing a fundamental role in programmed cell death. TX is a member of the ICE/CED-3 gene family encoding a cysteine protease that has a more than 50% sequence homology with ICE, especially in the region encoding the mature p20 and p10 ICE subunits and 30% sequence homology with Nedd-2/Ich-1L and CED-3. TX is able to cleave itself and the p30 ICE precursor and induces apoptosis in transfected cells1). TX is also a member of the caspase (CASP) family, CASP-4. An early biochemical event that occurs apoptosis in many cell types is the proteolytic cleavage of poly (ADP-ribose) polymerase (PARP), a nuclear enzyme involved in DNA repair. The several mammalian ICE homologues, ICE, TX, Nedd-2/Ich-1L and CPP32, are capable of cleaving PARP.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.

Human: 837 Mouse: 12363

Recombinant human TX (:1-270 a.a. terminal N)

This antibody reacts with caspase-4 (43 kDa) on Western blotting using total cell lysate from U937, HL60 and HUC-Fm (Human primary cultured fibroblast), and also reacts with 44 kDa of myc-tagged-TX expressed in 293T cell. Occasionally, unidentified 68 kDa band might be detected on western blotting in some cell lines.

1 year

This antibody was purified from hybridoma (clone 4B9) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with recombinant protein corresponding to N-terminal amino acids (1-270 aa) of human TX.

Caspase 4