Anti-DJ-1 (Human) mAb

WB - 1-10 ug/mL (chemiluminescence detection system)

DJ-1 (PARK7/CAP1/RS) was originally cloned as a putative oncogene capable of transforming NIH/3T3 cells in cooperation with h-ras, a protein expressed in sperm, and a regulator of RNA-protein interactions. DJ-1 has also been isolated as a gene associated with autosomal early-onset Parkinson's disease (PD). Taken together, DJ-1 appears to be involved in diverse biological processes. First, several lines of evidence suggest that DJ-1 plays a role in the oxidative stress response. In cultured mammalian cells, DJ-1 quenches reactive oxygen species and is converted into a variant with a more acidic isoelectric point. Therefore, DJ-1 protects against reactive oxygen species-induced cell death, and its suppression with small interfering RNA (siRNA) sensitizes cells to such insults. Second, DJ-1 modulates transcription through interaction with DJ-1-binding protein as well as with protein inhibitor of activated STAT (PIAS). The latter modulates the activity of various transcription factors. Third, DJ-1 has been recognized as a regulatory subunit of an RNA-binding protein. Fourth, DJ-1, which is structurally related to the molecular chaperone Hsp31, may have chaperone activity itself, preventing heat-induced aggregation of substrate proteins, including α-synuclein. In addition, several lines of evidence suggest that DJ-1 plays a role in human tumorigenesis. First, breast cancer patients have elevated levels of circulating DJ-1 and anti-DJ-1 autoantibodies compared to healthy and non-breast cancer patients. Secondly, DJ-1 protein is increased in primary non-small cell lung carcinoma samples. Thirdly, treatment of cells from the human lung cancer cell line NCI-H157 with paclitaxel and MEK inhibitor U0126 leads to a decrease in DJ-1 protein expression.

1 mg/mL

100 ug IgG in 100 ul PBS containing 50% glycerol, pH 7.2. Contains nopreservatives.

Human: 11315 Mouse: 57320

Full-length recombinant human DJ-1 (1-187 a.a.)

This antibody reacts with human DJ-1 (28 kDa) on Western blotting.

1 year

This antibody was purified from mouse ascites fluid using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with the recombinant full-length human DJ-1 (1-187 a.a.).

DJ-1