Anti-Myc-tag mAb-Magnetic Agarose
| Code | Size | Price |
|---|
| MBL-M047-10 | 20 Tests (Gel: 200 ul) | £323.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: PL14
Regulatory Status: RUO
Application: Immunoprecipitation (IP)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
IP - 10 uL of gel/400 uL of cell extract from 2 x 106 cells
Background:
Epitope tag technique has widely been accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation, and immunofluorescence, utilizing with an antibody that recognizes such an epitope. Myc-tag (EQKLISEEDL) sequence is widely used for the epitope tag, which corresponding to the partial peptide of human c-myc gene product.
Conjugate:
Magnetic Agarose
Formulation:
400 ug of antibody is covalently coupled to 200 ul of magnetic agarose gel and provided as400 ul gel slurry suspended in PBS/0.1% ProClin150
Immunogen Translated:
6myc-tagged fusion protein
Shelf Life:
1 year
Source:
Purified IgG from hybridoma supernatant
Target:
Myc
References
1) Sun, L., et al., J. Biol. Chem. 286, 4226-4235 (2011)
2) Arii, J., et al., Nature 467, 859-862 (2010)
Clone PL14 is used in these references.