Anti-Myc-tag mAb-Agarose

IP - 20 uL of gel slurry

Epitope tagging has widely been accepted technique that fuse an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-tag), EQKLISEEDL (Myc-tag) and YTDIEMNRLGK (VSV-G-tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.

Agarose

200 ug of anti-Myc-tag monoclonalantibody covalently coupled to 200 ul of agarose gel andprovided as a 50% gel slurry suspended in PBS containingpreservative (0.09% sodium azide) for a total volume of400 ul.

6myc-tag fusion protein

This antibody recognizes Myc-Tag peptide sequence on Immunoprecipitation.

1 year

This antibody was purified from hybridoma (clone PL14) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with 6myc-tag fusion protein.

Myc