Anti-Myc-tag mAb-Alexa Fluor 488
| Code | Size | Price |
|---|
| MBL-M047-A48 | 100 uL | £323.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1 κ
Antibody Clonality: Monoclonal
Antibody Clone: PL14
Regulatory Status: RUO
Applications:
- Flow Cytometry
- Immunocytochemistry (ICC)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
FCM - 10 ug/mL (final concentration) ICC - 5 ug/mL
Background:
Epitope tagging has widely been accepted technique that fuse an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VSV-G-Tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 1% BSA and 0.1% ProClin150.
Immunogen Translated:
6myc-tag fusion protein
Reactivity:
This antibody reacts with Myc-tag on Immunocytochemistry and Flow cytometry.
Shelf Life:
1 year
Source:
This antibody was purified from mouse ascites fluid using protein A agarose. This hybridoma (clone PL14) was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with 6myc-Tag fusion protein.
Target:
Myc
References
1)
Yabuta, N., et al., Nucleic Acid Res. 34, 4878-4892 (2006)
2)
Segawa, Y., et al., PNAS. 103, 12021-12026 (2006)
3)
Nagamori, I., et al., J. Biol. Chem. 281, 15073-15081 (2006)
4)
Sato, M., et al., FEBS J. 272, 6131-6144 (2005)
5)
Tsuda, H., et al., Clin. Cancer. Res. 11, 6880-6888 (2005)
6)
Yamashita, M., et al., J. Biol. Chem. 280, 29409-29419 (2005)
7)
Morizane, Y., et al., J. Biochem. 137, 125-132 (2005)
8)
Kojma, Y., et al., J. Biol. Chem. 279, 54032-54038 (2004)
9)
Morikawa, H., et al., Mol. Cell Biol. 24, 9401-9413 (2004)
10)
Muraki, M., et al., J. Biol. Chem. 279, 24246-24254 (2004)
11)
Turan, K., et al., Nucleic Acid Res. 32, 643-652 (2004)
12)
Hirota, K., et al., J. Biol. Chem. 278, 13056-13060 (2003)
13)
Tojo, M., et al., J. Biol. Chem. 277, 46576-46585 (2002)
14)
Ohya, T., et al., J. Biol. Chem. 277, 28099-28108 (2002)
15)
Sasaki, A., et al., J. Biol. Chem. 277, 22541-22546 (2002)
16)
Hamazaki, Y., et al., J. Biol. Chem. 277, 455-461 (2002)
17)
Nishida, T., et al., J. Biol. Chem. 276, 39060-39066 (2001)
18)
Fukumoto, T., et al., J. Biol. Chem. 276, 38441-38448 (2001)
19)
Yoshioka, K., et al., PNAS. 98, 7617-7622 (2001)
20)
Mukai, J., et al., J. Biol. Chem. 275, 17566-17570 (2000)
21)
Yoshizawa, T., et al., Hum. Mol. Genet. 9, 69-78 (2000)
22)
Itoh, M., et al., J. Biol. Chem. 274, 5981-5986 (1999)
Clone PL14 is used in these references.