Anti-MCM7 (Human) mAb
Code | Size | Price |
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MBL-M049-3 | 100 ug | £323.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 4B4
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunocytochemistry (ICC)
- Western Blot (WB)
Shipping:
4°C
Storage:
4°C
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Further Information
Applications:
WB - 1 ug/mL (chemiluminescence detection system) ICC - 5 ug/mL
Background:
The replication of the large genome of eukaryotic cells is made possible by the use of multiple replication origins per chromosome. However, multiple origins must be strictly regulated if all chromosomal sequences are to be replicated only once in S phase, and re-replication of DNA before completing mitosis is prohibited. This characteristic is thought to be due to the properties of ?initiation? proteins, such as Orc (Origin recognition complex), Cdc6 and MCM (Minichromosome maintenance) protein family. Experiments used with yeast and Xenopus had clearly shown that the replication preinitiation complex is assembled in an ordered sequence as Orc recruits Cdc6, which in turn recruits MCM complex (consisting of MCM 2-7 proteins). MCM proteins occur in high copy numbers in nuclei of human cells. They appear to be either free in the nucleoplasm or bound to chromatin. The fraction of structure-bound mammalian MCM proteins is highest at the beginning of S phase, but gradually decreases during progression of replication.
Formulation:
This antibody is lyophilized form.Prepare a stock solution by dissolving the lyophilized antibody in 100 ul of distilled water. After reconstitution, the IgG concentration should be 1 mg/mL in PBS (pH7.2)/1% sucrose. No preservative is contained.
Gene IDs:
Human: 4176 Mouse: 17220
Immunogen Translated:
Full-length recombinant human MCM7
Reactivity:
This antibody reacts with human and
mouse MCM7 on Western blotting and
Immunocytochemistry.
Shelf Life:
1 year
Source:
This antibody was purified from mouse
ascites fluid using protein A agarose. This hybridoma
(clone 4B4) was established by fusion of mouse myeloma
cell P3U1 with Balb/c mouse splenocyte immunized with
the recombinant full-length human MCM7.
Target:
Mcm7
References
1) Haraguchi, T., et al., J. Cell Sci. 120, 1967-1977 (2007)
2) Fujii-Yamamoto, H., et al., J. Biol. Chem. 280, 12976-
12987 (2005)
3) Ohsaki, E., et al., J. Virol. 78, 9936-9946 (2004)
4) Tatsumi, Y., et al., J. Biol. Chem. 278, 41528-41534 (2003)
5) Sato, N., et al., Genes Cells 8, 451-463 (2003)
6) Weinreich, M., et al., PNAS 96, 441-446 (1999)
7) Ritzi, M., et al., J. Biol. Chem. 273, 24543-24549 (1998)
8) Tanaka, T., et al., EMBO J. 17, 5182-5191 (1998)
9) Kubota, Y., et al., Cell 81, 601-609 (1995)
10) Chong, J., et al., Nature 375, 418-421 (1995)
11) Madline, M. A., et al., Nature 375, 421-424 (1995)
Clone 4B4 is used in reference number 1) - 5).