Anti-RCC1 (Human) mAb

WB - 1 ug/mL (chemiluminescence detection system) IP - 2 ug/200 uL of cell extract from 5 x 106 cells ICC - 5 ug/mL

The human RCC1 (Regulator of Chromosome Condensation) gene has been isolated as a gene that rescues tsBN2 mutation. The rcc1- phenotype is pleiotropic and includes G1 arrest, defects in mRNA splicing and mRNA transport. RCC1 is an abundant, highly conserved, chromatin-associated protein of 45 kDa which possesses an N-terminal region of 40 amino acid residues and an internal repeated domain in which about 60 amino acid residues are repeated seven times (RCC1 repeat). RCC1 is the principle mammalian guanine nucleotide exchange factor (GEF) for the nuclear G protein Ran, which GTPase activity is enhanced by GTPase-activating protein RanGAP located in the cytoplasm. RCC1 plays an important role in RNA transcription and processing, DNA replication, nuclear pore transport function and cell cycle regulation on Ran pathway. Epitope of this antibody is N-terminal domain (13-21 aa) of RCC1.

This antibody is lyophilized form. Prepare a stock solution by dissolving the lyophilized antibody in 100 ul of distilled water. After reconstitution, the IgG concentration will be 1 mg/mL in PBS (pH 7.2) / 1% sucrose. Contains no preservative.

Human: 1104 Mouse: 100088 Rat: 682908

Human full-length RCC1

This antibody reacts with human, mouse and rat RCC1 (45 kDa) on Western blotting, Immunoprecipitation and Immunocytochemistry. This antibody does not inhibit GEF activity of RCC1.

1 year

This antibody was purified from hybridoma (clone 3D11) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with the full-length human RCC1.

RCC1