Anti-Caspase-8 (Human) mAb

Product Code: MBL-M058-3

Supplier: MBL

Code	Size	Price

MBL-M058-3

100 ug

£296.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: IgG2b κ

Antibody Clonality: Monoclonal

Antibody Clone: 5D3

Regulatory Status: RUO

Target Species: Human

Applications:

Immunoprecipitation (IP)
Western Blot (WB)

Shipping:

4°C

Storage:

-20°C

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Documents

Datasheet

Further Information

Applications:

WB - 1 ug/mL IP - 1 ug/200 uL of cell extract from 2.5 x 10 ⁶ cells

Background:

Caspase-8 (FLICE/MACH/Mch5) is a member of the ICE (interleukin-1 converting enzyme)/CED-3 family cysteine protease. It is the most upstream protease that receives the activation signal from the Fas (APO1/CD95) and TNFR1 (Tumor Necrosis Factor Receptor 1) to initiate the apoptotic protease cascade that leads to activation of ICE/CED-3 family proteases. Caspase-8 has high homologous region to the ICE/CED-3 family in C-terminal and two death effecter domains (DED) in N-terminal. Binding of caspase-8 to FADD (MORT1) through association of their DED, and consequent activation of the caspases by their proteolytic cleavage, are thought to be critical steps in the initiation of Fas- and TNFR1-induced apoptosis. Recently the inhibitor of Fasand TNFR1-induced apoptosis is identified, called I-FLICE (FLIP/Casper/FLAME/CASH). I-FLICE has high homology to caspase-8 and it contains two DED, which interacts with caspase-8 and FADD, and potently inhibits Fas- and TNFR1-induced apoptosis.

Concentration:

1 mg/mL

Formulation:

1 mg/mL in PBS/50% glycerol

Gene IDs:

Human: 22861 Mouse: 12370

Immunogen Translated:

Recombinant human Caspase-8 (180-480 aa terminal C)

Reactivity:

This antibody reacts with caspase-8 on Western blotting with total cell lysate from human cell line.

Shelf Life:

1 year

Source:

This antibody was purified from mouse ascites fluid using protein A agarose. This hybridoma (clone 5D3) was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with the recombinant human caspases-8 corresponding to C-terminal amino acids (180-480 aa).

Target:

Caspase-8

References

1) Phillips, D. C., et al., Cancer Res. 67, 756-764 (2007) 2) Tsuruma, K., et al., J. Biol. Chem. 281, 11397-11404 (2006) 3) Martin, S., et al., Cancer Res. 65, 11447-11458 (2005) 4) Cryns, V., et al., Genes Dev. 12, 1551-1570 (1998) 5) Nicholson, D. W., et al., Trends Biochem. Sci. 22, 299-306 (1997) 6) Cohen, G. M., Biochem. J. 326, 1-16 (1997) 7) Muzio, M., et al., J. Biol. Chem. 272, 2952-2956 (1997) 8) Hu, S., et al., J. Biol. Chem. 272, 17255-17257 (1997) 9) Irmler, M., et al., Nature 388, 190-195 (1997) 10) Scaffidi, C., et al., J. Biol. Chem. 272, 26953-26958 (1997) 11) Duan, H., et al., J. Biol. Chem. 271, 16720-16724 (1996) 12) Boldin, M. P., et al., Cell 85, 803-815 (1996) 13) Muzio, M., et al., Cell 85, 817-827 (1996) 14) Arends, M. J., et al., Int. Rev. Exp. Pathol. 32, 223-254 (1991)