Anti-Caspase-10 (Human) mAb
| Code | Size | Price |
|---|
| MBL-M059-3 | 100 ug | £296.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1 κ
Antibody Clonality: Monoclonal
Antibody Clone: 4C1
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1~10 ug/mL
Background:
Apoptosis is a major form of cell death characterized by several morphological features that include chromatin condensation and fragmentation, cell membrane blebbing, and formation of apoptotic bodies. These morphological changes occur via signaling pathway that leads to the recruitment and activation of caspases, a family of cysteine-containing, aspartate-specific proteases. Caspases exist as inactive proenzymes in cells and are activated through their processing into two subunits in response to apoptotic stimulation. Activated caspases cleave a variety of important cellular proteins, other caspases, and Bcl-2 family members, leading to a commitment to cell death. Caspase-10 (also known as Mch4, FLICE2 and ICE-LAP4) is a ~58 kDa protein. This caspase acts upstream of the apoptosis induced cascade. Once this caspase is activated by certain apoptotic stimuli, this protein may be responsible for the activation of the other caspases such as caspase-3, -4, -7, -8, and -9. This antibody was made against human-originated immunogen, and detects human caspase-10 specifically.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 843
Immunogen Translated:
Recombinant human full-length FLICE2
Reactivity:
This antibody detects specifically 57 and 58 kDa of human caspase-10, 43 kDa and 30 kDa of human active caspase-10 on Western blotting*. This antibody reacts with isoforms caspase-10/a, 10/b and 10/d**.
This reactivity is reported in the reference number 8)* and 17)**.
Shelf Life:
1 year
Source:
This antibody was purified from mouse ascites fluid using protein A agarose. This hybridoma (clone 4C1) was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with the recombinant full-length of human FLICE2.
Target:
Caspase-10
References
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Milhas, D., et al., J. Biol. Chem. 280, 19836-19842 (2005)
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Clone 4C1 is used in reference 1) - 17).