Anti-Apaf-1 (Human) mAb

WB - 5 ug/mL

Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as other autoimmune diseases. Recently, the mammalian homologue of the key cell death gene CED-4 in C. elegans has been identified from human and mouse, and designated Apaf-1 (apoptosis protease activating factor 1) (Ref. 1,2). Apaf-1 binds to cytochrome c (Apaf-2), a reaction that requires dATP, and this leads to the formation of a large multimeric complex. The complex recruits pro-caspase-9, and activates caspase-9 (Apaf-3). Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 is ubiquitously expressed in human tissues (1).

1 mg/mL

Supplied as 100 micrograms of IgG in100 ul volume of PBS containing 50% glycerol.Contains no preservatives.

Human: 317 Mouse: 11783 Rat: 78963

His-fusion protein of human Apaf-1N-terminal region (1-421 aa)

This antibody detects ~130 kDa of human Apaf-1 protein from whole cell lysate of human cell lines, such as Jurkat, HeLa and U937 on Western blotting.

1 year

This antibody was purified from cell culture supernatant using protein-A Sepharose. This hybridoma was established by fusion of mouse myeloma P3U1 with Balb/c splenocyte immunized with His-tagged human Apaf-1 fusion protein corresponding to N-terminal amino acids (1-421 a. a.).

Apaf1