Anti-Podocalyxin (PCLP1) (Human) mAb
Code | Size | Price |
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MBL-M084-3 | 100 ug | £301.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 53D11
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Shipping:
4°C
Storage:
-20°C
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Further Information
Applications:
FCM - 10-20 ug/mL (final concentration)
Background:
Recent studies with avian embryos and murine embryonic stem cells have suggested that hematopoietic cells are derived from hemangioblasts, the common precursors of hematopoietic and endothelial cells. Hara et al. molecularly cloned podocalyxin-like protein 1 (PCLP1) as a novel surface marker for endothelial-like cells in the AGM (aorta-gonad-mesonephros) region of mouse embryos, where long-term repopulating hematopoietic stem cells (LTR-HSCs) are known to arise. PCLP1+ CD45- cells in the AGM region incorporated acetylated low-density lipoprotein and produced both hematopoietic and endothelial cells when cocultured with OP9 stromal cells. Moreover, multiple lineages of hematopoietic cells were generated in vivo when PCLP1+ CD45- cells were injected into neonatal liver of busulfan-treated mice. Today it is reported that the PCLP1 is identical with the Podocalyxin.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
Gene IDs:
Human: 5420 Mouse: 27205
Immunogen Translated:
CHO cells expressing the (length) human Podacalyxin/PCLP1
Reactivity:
This antibody reacts with human
Podocalyxin/PCLP1.
Shelf Life:
1 year
Source:
This antibody was purified from mouse
ascites fluid using protein A agarose beads. This
hybridoma was established by fusion of mouse myeloma
cell P3U1 with Balb/c splenocyte immunized with CHO
cell expressing full length human Podocalyxin/PCLP1.
Target:
PCLP1
References
1) Schopperle, WM., et al., Biochem. Biophys. Res.
Commun. 300, 285-290 (2003)
2) Doyonnas, R., et al., J. Exp. Med. 194, 13-27 (2001)
3) Hara, T., et al., Immunity 11, 567-78 (1999)
4) Kershaw, DB., et al., J. Biol. Chem. 272, 15708-15714
(1997)