Anti-Podocalyxin (PCLP1) (Human) mAb-PE

FCM - 20 uL (ready for use)

Recent studies with avian embryos and murine embryonic stem cells have suggested that hematopoietic cells are derived from hemangioblasts, the common precursors of hematopoietic and endothelial cells. Hara et al. molecularly cloned podocalyxin-like protein 1 (PCLP1) as a novel surface marker for endothelial-like cells in the AGM (aorta-gonad-mesonephros) region of mouse embryos, where long-term repopulating hematopoietic stem cells (LTR-HSCs) are known to arise. PCLP1+CD45- cells in the AGM region incorporated acetylated low-density lipoprotein and produced both hematopoietic and endothelial cells when cocultured with OP9 stromal cells. Moreover, multiple lineages of hematopoietic cells were generated in vivo when PCLP1+ CD45- cells were injected into neonatal liver of busulfan-treated mice. Today it is reported that the PCLP1 is identical with the Podocalyxin.

FITC

50 tests in 1 mL volume of PBS containing 1% BSA and 0.09% NaN3.

Human: 5420 Mouse: 27205

CHO cells expressing the (length) human Podocalyxin/PCLP1

This antibody reacts with human Podocalyxin/PCLP1 on Flow cytometry.

1 year

This antibody was purified from mouse ascites fluid using protein A agarose. This hybridoma (53D11) was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with CHO cell expressing full-length human Podocalyxin/PCLP1.

PCLP1