Anti-CD170 (Siglec-5) (Mouse) mAb

IP - 2 ug/300 uL of cell extract from 5x106 cells FCM - 5-10 ug/mL (final concentration)

SiglecE (sialic acid-binding immunoglobulin-like lectin E/SigLec5/CD170) is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. SiglecE is expressed on neutrophil and monocyte populations, both in the blood and bone marrow, as a dimeric, disulfide linked, 140 kDa type I membrane protein containing cytoplasmic immune receptor-based tyrosine signaling motifs. SiglecE recruits the SH2-domain-containing protein tyrosine phosphatases SHP-1 and SHP-2, which block signal transduction through dephosphorylation of signaling molecules. Thus SiglecE acts as an inhibitory receptor for ligand induced tyrosine phosphorylation.

1 mg/mL

100 ug IgG in 100 ul PBS containing 50% glycerol, pH 7.2. Contains no preservatives.

Human: 8778 Mouse: 83382

L cells transfected with mouse CD170

This antibody reacts with mouse SiglecE on Flow cytometry.

1 year

This antibody was purified from hybridoma (clone 8D2) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Wister rat lymph node immunized with mouse SiglecE transfected L cell.

Siglec-E