Anti-CD170 (Siglec-5) (Mouse) mAb-FITC
| Code | Size | Price |
|---|
| MBL-M096-4 | 50 ug (10-20 Assays) | £301.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 8D2
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Flow Cytometry
- Immunoprecipitation (IP)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
IP - 2 ug/300 uL of cell extract from 5x106 cells FCM - 25 ug/mL (final concentration)
Background:
SiglecE (sialic acid-binding immunoglobulin-like lectin E/SigLec5/CD170) is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. SiglecE is expressed on neutrophil and monocyte populations, both in the blood and bone marrow, as a dimeric, disulfide linked, 140 kDa type I membrane protein containing cytoplasmic immune receptor-based tyrosine signaling motifs. SiglecE recruits the SH2-domain-containing protein tyrosine phosphatases SHP-1 and SHP-2, which block signal transduction through dephosphorylation of signaling molecules. Thus SiglecE acts as an inhibitory receptor for ligand induced tyrosine phosphorylation.
Concentration:
50 ug/ml
Conjugate:
FITC
Formulation:
50 ug IgG in 1 mL PBS containing 1% BSA and 0.09% NaN3.
Gene IDs:
Human: 8778 Mouse: 83382
Immunogen Translated:
L cells transfected with mouse CD170
Reactivity:
This antibody reacts with mouse
SiglecE on Flow cytometry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 8D2) supernatant using protein G agarose. This
hybridoma was established by fusion of mouse myeloma
cell P3U1 with Wister rat lymph node immunized with
mouse SiglecE transfected L cell.
Target:
Siglec-E/CD170
References
1) Zhang, J.Q., et al., Eur. J. Immunol. 34, 1175-1184
(2004)