Anti-CD11c (Mouse) mAb-FITC
Code | Size | Price |
---|
MBL-M100-4 | 50 ug | £268.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 223H7
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Flow Cytometry
- Immunoprecipitation (IP)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
IP - 5 ug / 1000 uL of cell extract from
5x106 cells FCM - 10 ug/mL (final concentration)
Background:
The CD11c (αX integrin; ~150 kDa) glycoprotein non-covalently associates with CD18 (β2 integrin; ~95 kDa) to form the heterodimeric complement receptor type 4 (CR4), which is involved in monocyte/granulocyte adhesion during inflammatory responses. The CD11c/CD18 receptor binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. CD11c/CD18 is also implicated in B cell proliferation and mediates B cell binding to fibrinogen. CD11c is commonly used as a marker for dendritic cells, but it is also expressed on macrophages, monocytes, granulocytes, NK cells, activated T and B lymphocytes and microglia.
Concentration:
500 ug/ml
Conjugate:
FITC
Formulation:
50 ug IgG in 100 ul volume of
PBS containing 1% BSA and 0.09% NaN3.
Gene IDs:
Human: 3687 Mouse: 16411
Immunogen Translated:
Dendritic cells were isolated from C57BL / 6 mouse
Reactivity:
This antibody reacts with mouse CD11c
antigen on Flow cytometry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 223H7) supernatant using protein G agarose. This
hybridoma was established by fusion of mouse myeloma
cell Sp2/0-Ag14 with Wister rat lymphocyte immunized
with murine dendritic cells isolated from C57BL/6 mice.
Target:
CD11c
References
1) Fancke, B., et al., Blood Oct 2007;
10.1182/blood-2007-05-089292.
2) Kruger, T., et al., J. Am. Soc. Nephrol. 15, 613-21 (2004)
Clone 223H7 is used in reference number 1).