Anti-CD61 (gpIIIa) (Mouse) mAb
| Code | Size | Price |
|---|
| MBL-M109-3 | 100 ug | £280.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: 1-55-4
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Flow Cytometry
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1 ug/mL (chemiluminescence detection system) IP - 10 ug/200 uL of cell extract from
5 x 106 cells FCM - 10 ug/mL (final concentration)
Background:
Platelet activation results in a conformational change of the membrane spanning platelet integrin GPIIb/IIIa (αIIbβ3, CD41/CD61) enabling the binding of the plasma protein fibrinogen. This binding is primarily reversible, but it enhances platelet activation by outside-in signal causing receptor clustering, platelet secretion, and finally irreversible fibrinogen binding and platelet aggregation.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 3690 Mouse: 16416
Immunogen Translated:
Mouse platelet
Reactivity:
This antibody reacts with mouse CD61
antigen on Western blotting, Immunoprecipitation and Flow
cytometry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 1-55-4) supernatant using protein G agarose. This
hybridoma was established by fusion of mouse myeloma
cell P3U1 with Wister rat lymphocyte immunized with
mouse platelet.
Target:
CD61
References
1) Schwarz, M., et al., J. Pharmacol. Exp. Ther. 308, 1002-1011 (2004)
2) De Nichilo, M. O., et al., J. Immunol. 156, 284-288 (1996)