Anti-Atg3 (Human) mAb
Code | Size | Price |
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MBL-M133-3 | 100 ug | £346.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 3E8
Regulatory Status: RUO
Target Species:
- Hamster
- Human
- Mouse
- Rat
Applications:
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
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Further Information
Applications:
WB - 1 ug/mL (chemiluminescence detection system) IP - 2.5 ug/300 uL of cell extract from 3x106 cells ICC - 0.5 ug/mL
Background:
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Microtubule-associated protein light chain 3 (LC3) is a homologue of yeast Atg8, an essential component of autophagy. Following synthesis, the C-terminus of LC3 is cleaved by a cysteine
protease-Atg4, to produce LC3-I, which is located in cytosolic fraction. LC3-I is activated by the E1-like enzyme Atg7 and forms a Atg7-LC3-I thioester. Atg7-LC3-I is transferred to Atg3 to form Atg3-LC3-I thioester. Atg3 is an E2-like enzyme that catalyzes the conjugation of LC3-I and phosphatidylethanolamine (PE) to form LC3-II. The LC3-II-PE conjugate is essential for binding tightly to autophagosomal membrane.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 64422 Mouse: 67841 Rat: 171415
Immunogen Translated:
Recombinant human Atg3
Reactivity:
This antibody reacts with Atg3 on
Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 3E8) supernatant using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell P3U1 with Balb/c mouse lymphocyte immunized with
the recombinant human Atg3.
Target:
Atg3
References
1) Klionsky, D. J., et al., J. Cell Sci. 118, 7-18 (2005)
2) Tanida, I., et al., J. Biol. Chem. 277, 13739-13744 (2002)