Anti-GABARAP (Human) mAb
Code | Size | Price |
---|
MBL-M135-3 | 100 ug | £341.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 1F4
Regulatory Status: RUO
Target Species:
- Hamster
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Images
Documents
Further Information
Applications:
WB - 1 ug/mL (chemiluminescence detection system)
Background:
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Microtubule-associated protein light chain 3 (LC3) is a homologue of yeast Atg8, an essential component of autophagy. Following synthesis, the C-terminus of LC3 is cleaved by a cysteine
protease-Atg4, to produce LC3-I, which is located in cytosolic fraction. LC3-I is activated by the E1-like enzyme Atg7 and forms a Atg7-LC3-I thioester. Atg7-LC3-I is transferred to Atg3 to form Atg3-LC3-I thioester. Atg3 is an E2-like enzyme that catalyzes the conjugation of LC3-I and phosphatidylethanolamine (PE) to form LC3-II. The LC3-II-PE conjugate is essential for binding tightly to autophagosomal membrane.
Formulation:
1 mg/mL in PBS/50% glycerol, pH 7.2
Gene IDs:
Human: 11337 Mouse: 56486
Immunogen Translated:
Human GABARAP N-terminal
Reactivity:
This antibody reacts with GABARAP on
Western blotting.
Target:
GABARAP