Anti-His-tag mAb
| Code | Size | Price |
|---|
| MBL-M136-3 | 100 ug | £296.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 2D8
Regulatory Status: RUO
Application: Immunoprecipitation (IP)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
IP - 0.5-2 ug/sample
Background:
Expression vectors containing a protein and a tag peptide are commonly used. His-Tag fusion protein expression system is preferably used in various laboratories, because its simple protein purification step by heavy metal, such as nickel, affinity chromatography. This specific antibody for His-Tag fusion protein is useful tool for monitoring of the fusion protein expression and affinity purification.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Immunogen Translated:
6xHis-binding peptide carrier protein
Reactivity:
This antibody reacts with His-tagged
protein on Immunoprecipitation.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 2D8) supernatant using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell P3U1 with Balb/c mouse lymphocyte immunized with
the carrier protein conjugated 6xHis peptide.
Target:
His
References
1) Lee, J-H., et al., J. Virol. 80, 3844-3852 (2006)
2) Muro, S., et al., J. Cell Sci. 116, 1599-1609 (2003)
3) Isoyama, T., et al., J. Biol. Chem. 277, 39634-39641 (2002)
4) Isoyama, T., et al., J. Biol. Chem. 276, 21863-21869 (2001)
5) Toshima, J., et al., Mol. Biol. Cell 12, 1131-1145 (2001)