Anti-GFRα1 (Human) mAb

IP - 1 ug/100 uL of cell extract from 2 x 105 cells FCM - 10 ug/mL (final concentration)

Glial cell line-derived neurotrophic factor (GDNF) family plays a critical role in neurodevelopment and survival of central and peripheral neurons. The biological activity of GDNF family through receptor protein tyrosine kinase Ret and require a ligand specific co-receptor α (GFRα1, binds GDNF, whereas GFRα2 binds NRTN, GFRα3 binds ARTN and GFRα4 binds PSPN). Human GFRα1 is constituted from 465 amino acids and attach to the cell membrane by glycosylphosphatidylinositol (GPI) anchoring manner. It is widely accepted that homodimeric GDNF induce the complex formation of GFRα1 and further the stoichiometry of the signaling complex with GDNF2-GFRα12-Ret2, but the mechanism of complex formation remain unclear.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 2674 Mouse: 14585

Extracellular domain Recombinant human GFRα1 (24-440 aa)

This antibody reacts with GFRα1 on Immunoprecipitation and Flow cytometry.

1 year

This antibody was purified from mouse ascites fluid using protein A agarose. This hybridoma (clone 3H8) was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with recombinant human GFRα1 extracellular domain (24-440 aa).