Anti-Nuf2 (Human) mAb
| Code | Size | Price |
|---|
| MBL-M146-3 | 100 ug | £372.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 4H9
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 10 ug/mL (chemiluminescence detection system) IP - 1 ug/50 uL of cell extract from 5 x 106 cells
Background:
Nuf2, a widely conserved protein in eukaryote, compose the Ndc80 complex with NDC80/HEC1, SPC24 and SPC25. The Ndc80 complex is a constituent of the outer plate of kinetochore and acts to establish kinetochore-spindle connection at centromere for chromosome segregation in mitosis. Nuf2 localizes to kinetochores from late prophase to anaphase.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 83540 Mouse: 66977
Immunogen Translated:
Full-length recombinant human Nuf2
Reactivity:
This antibody reacts with Nuf2 (54 kDa)
on Western blotting and Immunoprecipitation.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 4H9) supernatant using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell P3U1 with C3H mouse lymphocyte immunized with
the recombinant full-length human Nuf2 (1-464 aa).
Target:
Nuf2
References
1) Ciferri, C., et al., J. Biol. Chem. 280, 29088-29095 (2005)
2) Wigge, P. A., et al., J. Cell Biol. 152, 349-360 (2001)
3) Janke, C., et al., EMBO J. 20, 777-791 (2001)
4) Osborne, M. A., et al., J. Cell Biol. 125, 853-866 (1994)