Anti-Borealin (Human) mAb
| Code | Size | Price |
|---|
| MBL-M147-3 | 100 uL | £345.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 1D11
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1 ug/mL (chemiluminescence detection system) IP - 1 ug/50 uL of cell extract from 1 x 106 cells ICC - 1-5 ug/mL
Background:
Borealin is a subunit of the chromosomal passenger complex (CPC), which is necessary for chromosome segregation and cytokinesis. While CPC shows a dynamic localization pattern during mitosis, Borealin and survivin, another subunit of CPC is essential to localize CPC at centromare from prophase to anaphase. It is thought that Borealin and survivin recruit aurora B, a kinase subunit of CPC, to inner centromare to allow phosphorylating appropriate substrates.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 55143 Mouse: 52276
Immunogen Translated:
Full-length recombinant human Borealin
Reactivity:
This antibody reacts with Borealin (35
kDa) on Western blotting, Immunoprecipitation and
Immunocytochemistry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 1D11) supernatant using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell P3U1 with C3H mouse lymphocyte immunized with
the recombinant full-length human Borealin (1-280 aa).
Target:
Borealin
References
1) Klein, U. R., et al., Mol. Biol. Cell 17, 2547-2558 (2006)
2) Gassmann, R., et al., J. Cell Biol. 166, 179-191 (2004)
3) Sampath, S. C., et al., Cell 118, 187-202 (2004)
4) Walker, M.G., Curr Cancer Drug Targets 1, 73-83 (2001)