Anti-LC3 (Human) mAb

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MBL
Product Code: MBL-M152-3
Product Group: Primary Antibodies
Supplier: MBL
CodeSizePrice
MBL-M152-3200 ug£374.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1 κ
Antibody Clonality: Monoclonal
Antibody Clone: 4E12
Regulatory Status: RUO
Target Species:
  • Hamster
  • Human
  • Mouse
  • Rat
Applications:
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
4°C
Storage:
-20°C

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Further Information

Applications:
WB - 5 ug/mL (chemiluminescence detection system) IP - 5 ug/300 uL of cell extract from 1x107 cells FCM - 40 ug/mL (final concentration) ICC - 40 ug/mL Other - Immuno-EM: 20 ug/mL
Background:
Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3.
Concentration:
2 mg/mL
Formulation:
200 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 81631, 84557 Mouse: 67443, 66734 Rat: 64862, 362245
Immunogen Translated:
Recombinant human LC3 (MAP1LC3B: 1-120 a.a.)
Reactivity:
This antibody reacts with LC3 (MAP1LC3A, B) on Immunocytochemistry, Western blotting and Immunoprecipitation.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 4E12) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with the recombinant human LC3 [MAP1LC3B (1-120 aa)].
Target:
LC3

References

1) Ho, H., et al., J. Biol. Chem. 286, 12509-12523 (2011) 2) Saiki, S., et al., Autophagy 7, 176-187 (2011) 3) Kasai, M., et al., J. Immunol. 183, 7278-7285 (2009) 4) Mookerjee, S., et al., J. Neurosci. 29, 15134-15144 (2009) 5) Nishitsuji, K., et al., Am. J. Pathol. 174, 957-969 (2009) 6) Wang, Y., et al., J. Biol. Chem. 283, 4766-4777 (2008) 7) Kabeya, Y., et al., J. Cell Sci. 117, 2805-2812 (2004) 8) Mizushima, N., et al., Mol. Biol. Cell 15, 1101-1111 (2004) 9) Mizushima, N., et al., J. Cell Biol. 152, 657-667 (2001) 10) Kabeya, Y., et al., EMBO J. 19, 5720-5728 (2000)