Anti-Atg5
| Code | Size | Price |
|---|
| MBL-M153-3 | 100 ug | £341.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1 κ
Antibody Clonality: Monoclonal
Antibody Clone: 4D3
Regulatory Status: RUO
Target Species:
- Hamster
- Human
- Mouse
Application: Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 2-5 ug/mL (chemiluminescence detection system)
Background:
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Autophagy has two ubiquitin-like conjugation systems, the Atg12 and LC3-II systems. In the Atg12 conjugation system, the Atg12-Atg5-Atg16L forms 800 kDa complex that elongates autophagic isolation membrane. After completion of the formation of the autophagosome, the Atg12-Atg5-Atg16L complex dissociates from the membrane.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 9474 Mouse: 11793
Immunogen Translated:
Full-length recombinant human Atg5 (275 a.a.)
Reactivity:
This antibody reacts with Atg5-Atg12 complex (55 kDa) on Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 4D3) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with recombinant full-length human Atg5 (275 aa).
Target:
Atg5
References
1)
Young, M., et al., J. Biol. Chem. in press
2)
Takaesu, G., et al., J. Biochem. 151, 157-166 (2012)
3)
Takahashi, Y., et al., Autophagy 7, 61-73 (2011)
4)
Hanada, T., et al., J. Biol. Chem. 282, 37298-37302 (2007)
5)
Pyo, J. O., et al., J. Biol. Chem. 280, 20722-20729 (2005)
6)
Mizushima, N., et al., J. Cell Biol. 152, 657-667 (2001)
This antibody is used in the reference 1)-3).