Anti-Atg12 (Human) mAb

WB - 1 ug/mL (chemiluminescence detection system) IP - 5 ug/250 uL of cell extract from 1x107 cells ICC - 10 ug/mL

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Autophagy has two ubiquitin-like conjugation systems, the Atg12 and LC3-II systems. In the Atg12 conjugation system, the Atg12-Atg5-Atg16L forms 800 kDa complex that elongates autophagic isolation membrane. After completion of the formation of the autophagosome, the Atg12-Atg5-Atg16L complex dissociates from the membrane.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 9140 Mouse: 67526

KLH-conjugated synthetic peptide (Human Atg12, internal reagion)

This antibody reacts with human Atg5-Atg12 complex (55 kDa) on Western blotting. Because almost all Atg12 exist in the form of Atg5-Atg12 complex, it is difficult to detect the monomeric Atg12.

1 year

This antibody was purified from hybridoma (clone 6E5) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with the KLH conjugated synthetic peptide corresponding to internal region of human Atg12.

Atg12