Anti-p62 (SQSTM1) (Human) mAb
| Code | Size | Price |
|---|
| MBL-M162-3 | 100 ul | £262.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1 κ
Antibody Clonality: Monoclonal
Antibody Clone: 5F2
Regulatory Status: RUO
Target Species: Human
Applications:
- Flow Cytometry
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1 ug/mL (chemiluminescence detection system) IP - 2 ug/250 uL of cell extract from 2.5x106 cells FCM - 2 ug/mL (final concentration) ICC - 5 ug/mL IHC - 2-10 ug/mL (Heat treatment required for paraffin)
Background:
p62/SQSTM1 interacts with various molecular groups such as RIP, TRAF6, ERK, aPKCs, and poly-ubiquitin through PB1 domain, Zn finger domain, and UBA domain. This protein directly interacts with LC3, which is localized on autophagosome membrane, and is degradated by autophagic-lysosome pathway. p62 regulates ubiquitin-positive protein aggregates caused by autophagy deficiency.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 8878 Mouse: 18412
Immunogen Translated:
Recombinant human p62 (120-440 a.a.)
Reactivity:
This antibody reacts with human p62 on Western blotting, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 5F2) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with the recombinant human p62 protein corresponding to amino acids 120-440.
Target:
p62
References
1) Ichimura, Y., et al., J. Biol. Chem. 283, 22847-22857 (2008)
2) Komatsu, M., et al., Cell 131, 1149-1163 (2007)