Anti-monomeric Kusabira-Orange 2 mAb

WB - 1 ug/mL (chemiluminescence detection system) IP - 5 ug/Sample ICC - 5 ug/mL IHC - 5 ug/mL (Frozen IH

CoralHue® Kusabira-Orange (KO) has been cloned from the stony coral, whose Japanese name is ?Kusabira-ishi?. Wild-type CoralHue® KO forms a brightly fluorescent dimer. CoralHue® KO has been carefully engineered to form a monomer, CoralHue® monomeric Kusabira Orange 1 (mKO1) that maintains the brilliance and pH stability of the parent protein. CoralHue® mKO2 is the mutant of mKO1 and has a feature of the rapid maturation. It absorbs light maximally at 551 nm and emits orange light at 565 nm. CoralHue® mKO2 can be used to label proteins or subcellular structures, or for reporter assay. M168-3 is available for immunostaining of ?Fucci-G1 Orange? (Fucci; Fluorescent Ubiquitination-based Cell Cycle Indicator). Fucci-G1 Orange encodes CoralHue® monomeric Kusabira-Orange2 (mKO2) fused to a part of human Cdt1 (hCdt1: Cdc10 dependent transcript 1). It is possible to use M168-3 for Fucci transgenic strain, B6.Cg-Tg(Fucci)596Bsi mice which express Fucci-G1 Orange.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Recombinant mKO2

This antibody reacts with monomeric Kusabira-Orange 2 on Western blotting, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.

1 year

This antibody was purified from hybridoma (clone 3B3) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with recombinant monomeric Kusabira-Orange 2.

Kusabira-Orange 2