Anti-GAPDH mAb
| Code | Size | Price |
|---|
| MBL-M171-3 | 100 ul | £301.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: 3H12
Regulatory Status: RUO
Target Species:
- Chicken (Gallus)
- Hamster
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 3 ug/mL (chemiluminescence detection system)
Background:
GAPDH (Glyceraldehyde- 3-phosphate Dehydrogenase) is a well-known enzyme, which catalyzes of glycolysis. GAPDH is stably and constitutively expressed at high levels in most tissues and cells, it is considered a housekeeping protein. Therefore, GAPDH is often used as a loading control of WB.
Concentration:
3 mg/mL
Formulation:
300 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 2597 Mouse: 14433
Immunogen Translated:
GAPDH from rabbit muscle
Reactivity:
This antibody reacts with GAPDH on Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 3H12) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with CH3 mouse lymphocyte immunized with the GAPDH from rabbit muscle.
Target:
GAPDH
References
1)
Tarze, A., et al., Oncogene 26, 2606-2620 (2007)
2)
Barber, R. D., et al., Physiol. Genomics. 21, 389-395 (2005)