Anti-α-Tubulin mAb
| Code | Size | Price |
|---|
| MBL-M175-3 | 100 ul | £269.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: 2F9
Regulatory Status: RUO
Target Species:
- Chicken (Gallus)
- Hamster
- Human
- Mouse
- Rat
Applications:
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 2 ug/mL (chemiluminescence detection system) IP - 5 ug/200 uL of cell extract from 2x106 cells ICC - 2 ug/mL
Background:
Microtubules are one of the components of the cytoskeleton, which performs essential and diverse functions within eukaryotic cells. Microtubules are composed of a heterodimer of α and β tubulins. Tubulin is a GTP-binding protein, and extension and shortening of the microtubules are regulated by binding/hydrolysis of GTP.
Concentration:
2 mg/mL
Formulation:
200 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 7846 Mouse: 22142 Rat: 64158
Immunogen Translated:
α-Tubulin N-terminal synthetic peptide of human
Reactivity:
This antibody reacts with human
α-tubulin on western blotting, Immunoprecipitation, and
Immunocytochemistry. The reactivity to mouse, rat,
hamster and chicken α-tubulin was confirmed by Western
blotting.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 2F9) supernatant using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell P3U1 with C3H mouse lymphocyte immunized with
the synthetic peptide corresponding to N-terminal of human
α-Tubulin.
References
1) Heald, R., and Nogales, E., J. Cell Sci. 115, 3-4 (2002)
2) Hall, J. L., and Cowan, N. J., Nucleic Acids Res. 13, 207-223 (1985)