Anti-EEA1 mAb
| Code | Size | Price |
|---|
| MBL-M176-3 | 100 ul | £269.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: 3C10
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1 ug/mL (chemiluminescence detection system) IP - 2 ug/200 uL of cell extract from 3x106 cells ICC - 1 ug/mL
Background:
The early endosome is a cellular compartment inside eukaryotic cells. It is receiving endocytosed material and sorting them for vesicular transport to late endosomes and lysosomes or for recycling to the plasma membrane. EEA1 (Early Endosome Antigen1) is a 170 kDa coiled-coil protein, which is required for vesicular transport of proteins through early endosomes. It binds membrane lipids through its FYVE domain.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 8411 Mouse: 216238 Rat: 314764
Immunogen Translated:
Terminal synthetic peptide of human EEA1N
Reactivity:
This antibody reacts with human EEA1
on western blotting, Immunoprecipitation, and
Immunocytochemistry. The reactivity to mouse and rat
EEA1 was confirmed by Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 3C10) supernatant using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell P3U1 with C3H mouse lymphocyte immunized with
the synthetic peptide corresponding to N-terminal of human
EEA1.
Target:
EEA1
References
1) Gaullier, J. M., et al., J. Biol. Chem. 275, 24595-24600 (2000)
2) Mu, F. T., et al., J. Biol. Chem. 270, 13503-13511 (1995)