Anti-beta-Actin mAb
| Code | Size | Price |
|---|
| MBL-M177-3 | 100 ul | £280.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1 κ
Antibody Clonality: Monoclonal
Antibody Clone: 6D1
Regulatory Status: RUO
Target Species:
- Chicken (Gallus)
- Hamster
- Human
- Mouse
- Rat
Applications:
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1 ug/mL (chemiluminescence detection system) IP - 2 uL/200 uL of cell extract from 2 x 106 cells
Background:
Actin is a 42 kDa protein found in eukaryotic cells. Actin is also one of the most highly conserved proteins. Actin participates in many important cellular activities including muscle contraction, cellmovement, cell division and the formation of the cytoskeleton. The beta actin exists in most cell types as components of the cytoskeleton.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Immunogen Translated:
Synthetic peptide N-terminal β-Actin
Reactivity:
This antibody reacts with human β-actin
on western blotting and Immunoprecipitation. The
reactivity to mouse, rat, hamster and chicken β-actin was
confirmed by Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 6D1) supernatant using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell P3U1 with C3H mouse lymphocyte immunized with
KLH conjugated synthetic peptide, corresponding to
N-terminus of β-actin.
References
1) Watanabe-Nakayama,T., et al.,Biol.Open2,667-674(2013) [WB]
2) Vandekerckhove, J. and Weber, K., PNAS 75, 1106 (1978)