Anti-Calnexin mAb
| Code | Size | Price |
|---|
| MBL-M178-3 | 100 ul | £280.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: 4F10
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 0.1 ug/mL (chemiluminescence detection system) IP - 1 ug/300 uL of cell extract from 3 x 106 cells ICC - 1 ug/mL
Background:
The endoplasmic reticulum (ER) is a eukaryotic organelle, which serves many general functions, including the facilitation of protein folding. Calnexin is a 90 kDa integral membrane protein of the ER. Calnexin is one of the chaperone proteins, which play a major role in the quality control of the ER by the retention of incorrectly folded proteins.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Immunogen Translated:
N-terminal synthetic peptide Calnexin
Reactivity:
This antibody reacts with human
Calnexin for Western blotting, Immunoprecipitation and
Immunocytochemistry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 4F10) supernatant using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell P3U1 with C3H mouse lymphocyte immunized with
synthetic peptide corresponding to N-terminus of human
Calnexin.
Target:
Calnexin
References
1) Kleizen, B., and Braakman, I., Curr. Opin. Cell Biol. 16, 343-349 (2004)
2) David, V., et al., J. Biol. Chem. 268, 9585-9592 (1993)