Anti-GM130 mAb

MBL
Product Code: MBL-M179-3
Product Group: Primary Antibodies
Supplier: MBL
CodeSizePrice
MBL-M179-3100 ul£336.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: 5G8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
4°C
Storage:
-20°C

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Further Information

Applications:
WB - 1 ug/mL (chemiluminescence detection system) IP - 2 ug/300 uL of cell extract from 3 x 106 cells ICC - 5 ug/mL
Background:
The Golgi apparatus is a eukaryotic organelle, which is mainly devoted to processing the proteins synthesized in the endoplasmic reticulum (ER). GM130 is a member of the golgin family of coiled-coil proteins that localizes predominantly to the cis-Golgi. GM130 might participate in ER-Golgi traffic.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 2801
Immunogen Translated:
GM130 C-terminal synthetic peptide
Reactivity:
This antibody reacts with human GM130 on Western blotting, Immunoprecipitation, and Immunocytochemistry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 5G8) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with the synthetic peptide corresponding to C-terminal of human GM130.
Target:
GM130

References

1) Diao, A., et al., J. Biol. Chem. 283, 6957-6967 (2008) 2) Alvarez, C., et al., J. Biol. Chem. 276, 2693-2700 (2001)