Anti-KDEL mAb
| Code | Size | Price |
|---|
| MBL-M181-3 | 100 ul | £296.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: 1D5
Regulatory Status: RUO
Target Species:
- Hamster
- Human
- Mouse
- Rat
Applications:
- Immunocytochemistry (ICC)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 0.1 ug/mL (chemiluminescence detection system) ICC - 0.5 ug/mL
Background:
The KDEL (lys-asp-glu-leu) sequence is most common endoplasmic reticulum (ER) retention signal. The ER resident proteins, which should be located in the ER, have this retention motif, and this retention is mediated by KDEL receptor.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Immunogen Translated:
Mouse GRP78 C-terminal 644-655 aa
Reactivity:
This antibody reacts with human
proteins containing KDEL sequence on Western blotting
and Immunocytochemistry. The reactivity to mouse, rat and
hamster proteins containing KDEL sequence was
confirmed by Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 1D5) supernatant using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell P3U1 with C3H mouse lymphocyte immunized with
KLH conjugated synthetic peptide CTGEEDTSEKDEL
corresponding to the amino acid residues 644-655 of mouse
GRP78.
Target:
KDEL
References
1) Pelham, H. R., EMBO J. 7, 913-918 (1988)
2) Munro, S., et al., Cell 13, 899-907 (1987)