Anti-LC3 mAb-HRP-DirecT
| Code | Size | Price |
|---|
| MBL-M186-7 | 50 ul | £357.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: 8E10
Regulatory Status: RUO
Target Species:
- Hamster
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
WB - 1:1,000
Background:
Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3.
Conjugate:
HRP
Formulation:
PBS/Preservative/Stabilizer
Immunogen Translated:
Human LC3 (MAP1LC3B), 1-120 aa (recombinant)
Target:
LC3
References
1) Jia, W., et al., J. Immunol. 186, 5313-5322 (2011)
2) Tabata, K., et al., Mol. Biol. Cell 21, 4162-4172 (2010)
3) Mookerjee, S., et al., J. Neurosci. 29, 15134-15144 (2009)
4) Saitoh, T., et al., Nature 456, 264-268 (2008)
5) Kabeya, Y., et al., J. Cell Sci. 117, 2805-2812 (2004)
6) Mizushima, N., et al., Mol. Biol. Cell 15, 1101-1111 (2004)
7)Mizushima, N., et al., J. Cell Biol. 152, 657-668 (2001)