Anti-Np95 (Uhrf1) mAb
| Code | Size | Price |
|---|
| MBL-M202-3 | 100 ul | £291.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2c κ
Antibody Clonality: Monoclonal
Antibody Clone: R32-44
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunocytochemistry (ICC)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1 ug/mL ICC - 3 ug/mL
Background:
DNA methylation inheritance is the process of copying pre-existing methylation patterns onto newly replicated DNA strand after DNA replication. Dnmt1 is the maintenance DNA methyltransferase, which methylates hemimethylated CpGs that appear after DNA repairs and replications. The mouse Np95, also known as Uhrf1, is a nuclear protein, which contains an ubiquitin-like domain, a tandem Tudor domain, a PHD (Plant Homeodomain) finger, an SRA (SET-and RING-associated) domain, and a RING (Really Interesting New Gene) finger domain. Np95 recognizes hemimethylated CpGs via the SRA domain, and recruits Dnmt1 to the sites to transfer the methylation patterns onto the newly synthesized DNA strand. Therefore, the interaction between Np95 and Dnmt1 is necessary for the inheritance of DNA methylation.
Formulation:
PBS/50% glycerol, pH 7.2
Gene IDs:
Human: 29128 Mouse: 18140
Immunogen Translated:
Recombinant protein, corresponding to amino acids 590-713 of mouse Np95 (Uhrf1)
Shelf Life:
1 year
Source:
Purified IgG from hybridoma supernatant
Target:
Np95
References
1) Hashimoto, H., et al., Nature 455, 826-829 (2008)
2) Arita, K., et al., Nature 455, 818-821 (2008)
3) Bostick, M., et al., Science 317, 1760-1764 (2007)
4) Sharif, J., et al., Nature 450, 908-912 (2007)
5) Unoki, M., et al., Oncogene 23, 7601-7610 (2004)