Anti-PI-3 Kinase (p85alpha) (Human) mAb

WB - 1 ug/mL (chemiluminescence detection system) IP - 1-5 ug/600 uL of cell extract from 5x10cells ICC - 10 ug/mL

Phosphoinositide 3-kinase (PI3K) family of enzymes generate 3-phosphorylated phosphoinositides that act as second messengers downstream of numerous cellular receptors. This family control several cellular responses including cell growth, survival cytoskeletal remodeling and the trafficking of intracellular organelles in many different cell types. There are four subgroups (class IA, IB, II, III) of PI3K with distinct structure and substrate specificity. Class IA PI3K, which function downstream of activated tyrosine kinases, are heterodimers composed of a p110 catalytic subunit (α, β, or δ) and a tightly associated regulatory subunit (p85α, p55α, p50α, p85β, or p55γ). Each regulatory subunit can interchangeably associate with different catalytic subunits and shows a unique tissue distribution. p85α is the major regulatory subunit of class IA PI3Ks in most types of cells including immune cells.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 5266 Mouse: 20702 Rat: 25513

Recombinant human p85α

This antibody reacts with p85β, which is regulatory subunit of PI3-kinase on Western blot, Immunoprecipitation and Immunocytochemistry. This antibody recognizes mutant p85β protein expressed in E. coli to be possessed in amino acid position '1-79' described below. This antibody does not react with p85β.

1 year

This antibody was purified from mouse ascites fluid using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell SP2/0-Ag14 with Balb/c mouse splenocyte immunized with recombinant human p85α.

PI-3