Anti-Cyclin D1 (Human) mAb

WB - 1 ug/mL (chemiluminescence detection system) IP - 1-10 ug/200 ug of cell extract from 5x106 cells FCM - 10 ug/mL (final concentration) IHC - 1-10 ug/mL (Heat treatment required for paraffin)

Cyclin D1, also known as Bcl-1, CCND1, or PRAD-1, is a 36 kDa nuclear protein that plays a key regulatory role during the G1 phase of the cell cycle by regulating the activity of Cdk proteins. Cyclin D1 forms a complex with Cdk4, then binds to and phosphorylates Rb protein, triggering cells to progress from G0/G1 to S and thus driving cellular proliferation. Cyclin D1 protein overexpression is found in the majority of human breast cancers and is usually linked with poor prognosis. Overexpression of cyclin D1 is a well-established criterion for the diagnosis of Mantle Cell Lymphoma, and also has been used to distinguish malignant breast carcinomas from premalignant breast lesions.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 595 Mouse: 12443

Recombinant human PRAD1/cyclinD1

This antibody reacts with human Cyclin D1 (36 kDa) on Western blotting, Immunoprecipitation, Immunohistochemistry and Flow cytometry. This clone 5D4 recognizes human Cyclin D1, D2 and mouse Cyclin D1, D2, but not human and mouse Cyclin D3.

1 year

This antibody was purified from hybridoma (clone 5D4) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell PAI with Balb/c mouse splenocyte immunized with the recombinant human PRAD1/Cyclin D1.

Cyclin D1