Anti-PCNA (Proliferating Cell Nuclear Antigen) (Rat) mAb
| Code | Size | Price |
|---|
| MBL-MH-12-3 | 100 ug | £323.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1 κ
Antibody Clonality: Monoclonal
Antibody Clone: 5A10
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Flow Cytometry
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
WB - 5 ug/mL IP - 5 ug/200 uL of cell extract from 2.5 x 10 6 cells FCM - 10-50 ug/mL (final concentration) ICC - 10 ug/mL IHC - 2 ug/mL (Heat treatment required for paraffin)
Background:
The proliferating cell nuclear antigen
(PCNA) also known as cyclin, that appearance correlates
with the proliferative state of the cells. PCNA level
increases 2 to 3 times between early G1 and S phases, it
shows maximum being during S phase and declines during
G2 and M phases. It is revealed to be a co-factor of DNA
polymerase ? and to be necessary for DNA synthesis and
cell cycle progression. Examination of PCNA is important
in the malignant tumor research.
Concentration:
1 mg/mL
Formulation:
1 mg/mL in PBS/50% glycerol, pH 7.2
Gene IDs:
Human: 5111 Mouse: 18538
Immunogen Translated:
Recombinant rat PCNA
Reactivity:
This antibody reacts with human, mouse
and rat PCNA on Western blotting, Immunoprecipitation,
Flow cytometry, Immunohistochemistry and
Immunocytochemistry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 5A10) supernatant using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell P3U1 with Balb/c mouse splenocyte immunized with
recombinant rat PCNA.
Target:
PCNA
References
Tsuji, Y., et al., Genes Cells 13, 343-354 (2008)
Haraguchi, T., et al., J. Cell Sci. 120, 1967-1977 (2007) Kudoh, A., et al., J. Virol. 78, 104-115 (2004)
Shiomi, Y., et al., Genes Cells 9, 279-290 (2004)
Fujita, K., et al., Genes Cells 8, 559-571 (2003)
Suga, S., et al., J. Am. Soc. Nephrol. 14, 397-406 (2003)
Iida, T., et al., Genes Cells 7, 997-1007 (2002)
Ibe, S., et al., Genes Cells 6, 815-824 (2001)
Fujita, N., et al., Am. J. Physiol. Heart Circ. Physiol. 277, 515-523 (1999)
Miyachi, K., et al., J. Immunol. 121, 2228-2234?1978?
Prelich, G., et al., Nature 326, 517-520?1987?
Matsumoto, K., et al., EMBO J. 6, 637-642 (1987)