Anti-Ubiquitin mAb

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MBL
Product Code: MBL-MK-12-3
Product Group: Primary Antibodies
Supplier: MBL
CodeSizePrice
MBL-MK-12-3100 ug£323.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 2C5
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
4°C
Storage:
-20°C

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Further Information

Applications:
WB - 5 ug/mL (chemiluminescence detection system) IP - It is reported that 2C5 can be used in this application in the reference number 4. ICC - It is reported that 2C5 can be used in this application in the reference number 3).
Background:
Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. There are several reports showed that intracellular abnormal and short-lived proteins are degraded through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Immunogen Translated:
Ubiquitin bovine erythrocytes
Reactivity:
This antibody reacts with human, mouse, rat and bovine ubiquitin on Western blotting. Clone 2C5 does not react with multi ubiquitin. Clone 2C5 and 1B3 (MBLI; code no. MK-11-3) recognize different epitope sites each other.
Shelf Life:
1 year
Source:
This antibody was purified from ascites fluid using protein A agarose. This hybridoma (clone 2C5) was established by fusion of mouse myeloma cell P3U1 with F1 mouse (C57BL/6 x Balb/c) splenocyte immunized with the bovine erythrocyte ubiquitin.
Target:
Ubiquitin

References

1) Ishioka, T., et al., Genes Cells 12, 735-744 (2007) 2) Ott, D. E., et al., J. Virol. 77, 3384-3393 (2003) 3) Sutovsky, P., et al., Biol. Reprod. 63, 582-590 (2000) 4) Hiyama, H., et al., J. Biol. Chem. 274, 28019-28025 (1999) Clone 2C5 is used in these references.