Anti-Ash (Grb2) mAb
| Code | Size | Price |
|---|
| MBL-MS-20-3 | 100 ul | £296.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1 κ
Antibody Clonality: Monoclonal
Antibody Clone: 3F2
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunofluorescence (IF)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 2-10 ug/mL (chemiluminescence detection system) IF - This antibody can be used in this application in the reference number 1).
Background:
Grb2 (Growth factor receptor bound protein 2) also known as Ash, is a 25-28 kDa molecule composed of central Src homology (SH2) domain and SH3 domains. Grb2 associates with tyrosine phosphorylated proteins such as EGFR, PDGFR, IRS-1, Shc and Gab1 via its Sh2 domain. The SH3 domain of Grb2 binds to Sos, which stimulates the GTP binding activity of Ras, cause activation of MAP kinase and other signaling pathways. Grb2 is an adaptor protein, which does not have enzymatic activity itself. Grb2 express ubiquitously and has two isoforms.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul PBS containing 50% glycerol, pH 7.2. Contains no preservatives.
Gene IDs:
Human: 2885 Mouse: 14784
Immunogen Translated:
Recombinant Ash/Grb-2
Reactivity:
This antibody reacts with N-terminal SH3 domain of Ash/Grb2 on Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from mouse ascites fluid by ammonium sulfate precipitation and affinity chromatography on protein A agarose. This hybridoma (clone 3F2) was established by fusion of mouse myeloma cell P3U1with Balb/c splenocyte immunized with the recombinant Ash/Grb2.
Target:
Ash/Grb-2
References
1) Shinoda, T., et al., J. Neurosci. 27, 4-14 (2007)
2) Ueno, H., et al., J. Biol. Chem. 272, 8739-8742 (1997)
3) Ueno, H., et al., J. Biol. Chem. 271, 27707-27714 (1996)
4) Ueno, H., et al., J. Biol. Chem. 270, 20135-20142 (1995)
5) Wada, H., et al., Cancer Res. 55, 3192-3196 (1995)
6) Odai, H., et al., J. Biol. Chem. 270, 10800-10805 (1995)
7) Odai, H., et al., Blood 89, 2745-2756 (1997)
8) Matuoka, K., et al., EMBO J. 12, 3467-3473 (1995)
9) Rozakis-Adcock, M., et al., Nature 363, 83-85 (1993)
10) Li, N., et al., Nature 363, 85-88 (1993)
11) Gale, N. W., et al., Nature 363, 88-92 (1993)
12) Matuoka, K., et al., PNAS 89, 9015-9019 (1992)
13) Lowenstein, E. J., et al., Cell 70, 431-442 (1992)
14) Clark, S. G., et al., Nature 356, 340-344 (1992)
15) Williams, L. T., Current Biology 11, 601-603 (1992)
Clone 3F2 is used in reference number 1)-7).