Anti-ATBF1 (D1-120) (Human) pAb

MBL
Product Code: MBL-PD010
Product Group: Primary Antibodies
Supplier: MBL
CodeSizePrice
MBL-PD010100 ul£323.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
4°C
Storage:
-20°C

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Documents

Further Information

Applications:
WB - 1:2000 (chemiluminescence detection system) IP - 1 uL/150 ug of cell lysate ICC - 1?500 IHC - 1:2500-1:5000 (DAB staining) IF - 1:500
Background:
ATBF1 (also known as ZFHX3) is a transcription factor that has two-protein isoforms, the 404 kDa ATBF1-A and the 306 kDa ATBF1-B. ATBF1-A contains four homeodomains and 23 zinc-finger motifs. ATBF1-B contains four homeodomains and 18 zinc fingers. ATBF1 is identified as DNA-binding protein, which binds to an AT-rich element of the human α-fetoprotein (AFP) gene, as a result suppressing its transcription activity. ATBF1 is also involved in cell cycle arrest and cooperating with p53 to activate the p21Waf1/Cip1 promoter. ATBF1 is expressed in the differentiation fields in association with β-tubulin III and MAP2 that are the neuronal differentiation marker. ATBF1 plays a crucial role in neuronal development and cell cycle arrest.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains no preservative.
Gene IDs:
Human: 463 Mouse: 11906 Rat: 307829
Immunogen Translated:
Internal area of ??human ATBF1 (2107-2147 aa). Internal region of the mouse will match and ATBF1 (2114-2154 aa).
Reactivity:
This antibody reacts with internal region of ATBF1 on Western blotting, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum. The rabbit was immunized with recombinant region of human ATBF1 (2107-2147 aa.) corresponding to the identical amino acid sequence of mouse ATBF1 (2114-2154 aa.).
Target:
ATBF1

References

1) Jung, C. G., et al., Development 132, 5137-5145 (2005) 2) Zhang, Z., et al., Clin. Cancer Res. 11, 193-198 (2005) 3) Ishii, Y., et al., J. Comp. Neurol. 465, 57-71 (2003) 4) Berry, F. B., et al., J. Biol. Chem. 276, 25057-25065 (2001) 5) Miura, Y., et al., J. Biol. Chem. 270, 26840-26848 (1995) This antibody is used in the reference number 1).