Anti-LC3 (Rat) pAb
| Code | Size | Price |
|---|
| MBL-PD014 | 100 ul | £374.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Hamster
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:1000
Background:
Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 84557 Mouse: 66734 Rat: 64862
Immunogen Translated:
Full-length recombinant rat LC3
Reactivity:
This antibody reacts with LC3-I (18
kDa) and LC3-II (16 kDa) on Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum
using protein A agarose. The rabbit was immunized with
the recombinant full-length rat LC3.
Target:
LC3
References
1) Kabeya, Y., et al., J. Cell Sci. 117, 2805-2812 (2004)
2) Mizushima, N., et al., Mol. Biol. Sci. 15, 1101-1111 (2004)
3) Mizushima, N., et al., J. Cell Biol. 152, 657-667 (2001)
4) Kabeya, Y., et al., EMBO J. 19, 1720-5728 (2000)
This antibody is used in these references.