Anti-Atg14 (Human) pAb
| Code | Size | Price |
|---|
| MBL-PD026 | 100 ul | £470.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Affinity Purified Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:500 (chemiluminescence detection system) IP - 5 uL/300 uL of cell extract from 3x106 cells
Background:
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Mammalian homologues of Atg14/Atg14L/BARKOR localizes on the isolation membrane and autophagosome, and it necessary for autophagosome formation. Atg14 binds to the PI3K complex (Beclin-1, Vps34, Vps15) and promotes the autophagosome formation.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 22863 Mouse: 100504663 Rat: 305831
Immunogen Translated:
Recombinant human Atg14 (167-404 a.a.)
Reactivity:
This antibody reacts with human, mouse and rat Atg14 on Western blotting and Immunoprecipitation.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with recombinant human Atg14 (167-404 aa).
Target:
Atg14
References
1)
Matsunaga, K., et al., Nat. Cell Biol. 11, 385-396 (2009)
2)
Zhong, Y., et al., Nat. Cell Biol. 11, 468-476 (2009)
3)
Itakura, E., et al., Mol. Biol. Cell 19, 5360-5372 (2008)
4)
Sun, Q., et al., PNAS 105, 19211-19216 (2008)
This antibody is used in the reference number 3).