Anti-Rubicon (Human) pAb
| Code | Size | Price |
|---|
| MBL-PD027 | 100 uL | £374.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Affinity Purified Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:1000 (chemiluminescence detection system) IP - 5 uL/300 uL of cell extract from 3x106 cells
Background:
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome /vacuole for degradation. Rubicon was identified as Beclin1 interacting protein. Three distinct Beclin1 complexes exist in cells, one of the complexes including Rubicon (Beclin1, hVps34, hVps15, UVRAG, Rubicon) down regulates the process of autophagosome maturation and endocytosis.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 9711
Immunogen Translated:
End Recombinant human RubiconC (722-972 aa)
Reactivity:
This antibody reacts with human Rubicon on Western blotting and Immunoprecipitation.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with recombinant C terminus of human Rubicon (722-972 aa).
Target:
Rubicon
References
1)
Matsunaga, K., et al., Nat. Cell Biol. 11, 385-396 (2009)
2)
Zhong, Y., et al., Nat. Cell Biol. 11, 468-476 (2009)
This antibody is used in the reference number 1).