Anti-Atg2A pAb
| Code | Size | Price |
|---|
| MBL-PD041 | 100 ul | £335.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Affinity Purified Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Hamster
- Human
- Mouse
- Rat
Applications:
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:1,000 IP - 5 uL/300 uL of cell extract from 3 x 106 cells ICC - 1:400
Background:
Yeast Atg2 localizes to a perivacuolar punctate structure termed the preautophagosomal structure (PAS), where autophagosome formation is initiated5). Recruitment of Atg2 to the PAS requires the Atg1 complex, the PtdIns3-kinase complex, and Atg9. Atg2 interacts with Atg18, a PtdIns(3)P-binding protein, and Atg9. Atg2 and Atg18 are required for retrograde transport of Atg9 from the PAS. In mammals, there are two Atg2 homologues, Atg2A and Atg2B, both of which are involved in autophagosome formation1)
Formulation:
PBS containing 50% Glycerol (pH 7.2). No preservative is contained.
Gene IDs:
Human: 23130 Mouse: 329015 Rat: 689688
Immunogen Translated:
Human Atg2A, 700 aa-1,400 aa (recombinant)
Shelf Life:
1 year
Source:
Purified Ig from rabbit serum
Target:
Atg2A
References
1) Velikkakath, A. K., et al., Mol. Biol. Cell 23, 896-909 (2012) [WB, IC]
2) Suzuki, K., et al., Genes Cell 12, 209-218 (2007)
3) Suzuki, K., et al., EMBO J. 20, 5971-5981 (2001)
4) Str?mhaug, P. E., et al., J. Biol. Chem. 276, 42422-42435 (2001)
5) Shintani, T., et al., J. Biol. Chem. 276, 30452-30460 (2001)
6) Wang, C. W., et al., J. Biol. Chem. 276, 30442-30451 (2001)